Cyclic AMP-dependent protein kinase does not phosphorylate cyclic GMP-dependent protein kinase in vitro
نویسندگان
چکیده
منابع مشابه
Cyclic GMP-dependent Protein Kinase*
Cyclic GMP (cGMP) inhibits intracellular calcium ([Ca2+Ii) mobilization in vascular smooth muscle cells by a mechanism that is not well understood. Because several studies suggest that cGMP inhibits inositol 1,4,5trisphosphate (IP,) action, we examined the effects of cGMP-dependent protein kinase on IP, receptor phosphorylation. The purified IP, receptor was phosphorylated using either the cGMP...
متن کاملby cyclic AMP-dependent protein kinase
Myosin light-chain kinase was purifed from bovine carotid artery. Approx. 90% of myosin kinase was extracted in the supernatant fraction with buffer containing EDTA during myofibril preparation. The soluble fraction yielded two distinct peaks on DEAE-Sephacel chromatography. Peak I was eluted at a conductance of 11-12mmho and was completely dependent on Ca2+-calmodulin for its activity. Peak II...
متن کاملEzrin is a cyclic AMP-dependent protein kinase anchoring protein.
cAMP-dependent protein kinase (A-kinase) anchoring proteins (AKAPs) are responsible for the subcellular sequestration of the type II A-kinase. Previously, we identified a 78 kDa AKAP which was enriched in gastric parietal cells. We have now purified the 78 kDa AKAP to homogeneity from gastric fundic mucosal supernates using type II A-kinase regulatory subunit (RII) affinity chromatography. The ...
متن کاملPhosphorylation of tyrosine hydroxylase by cyclic GMP-dependent protein kinase.
Tyrosine hydroxylase purified from rat pheochromocytoma was phosphorylated and activated by purified cyclic GMP-dependent protein kinase as well as by cyclic AMP-dependent protein kinase catalytic subunit. The extent of activation was correlated with the degree of phosphate incorporated into the enzyme. Comparable stoichiometric ratios (0.6 mol phosphate/mol tyrosine hydroxylase subunit) were o...
متن کاملCyclic AMP-Dependent Protein Kinase in Atrial Cells
Cholinergic and f-adrenergic stimulations of ionic currents are major physiological mechanisms in the regulation of heart rate and contractility. Muscarinic receptor stimulation is known to reduce f-adrenergic effects on calcium current via reduction of cyclic AMP. Whether the P-adrenergic stimulation affects the muscarinic response is not known. I report here that the f8-adrenergic agonist iso...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1983
ISSN: 0014-5793
DOI: 10.1016/0014-5793(83)80345-7